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JoVE Journal Biochemistry
Stegvis doseringsprotokoll för ökat dataflöde i etikettfria Impedansbaserade GPCR-analyser
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Stegvis doseringsprotokoll för ökat dataflöde i etikettfria Impedansbaserade GPCR-analyser

Article DOI: 10.3791/60686-v • 06:13 min • February 21st, 2020
February 21st, 2020 •
1, 1, 1, 1, 2, 2, 3, 3, 1,4
1Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 2Institute of Pharmacy, University of Regensburg, 3Department of Chemistry and Pharmacy, Friedrich-Alexander University Erlangen-Nuernberg, 4Fraunhofer Research Institution for Microsystems and Solid State Technologies EMFT

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Detta protokoll visar realtidsregistrering av full dosresponsrelationer för agonist-inducerad GPCR-aktivering från ett enda cellskikt som odlas på en enda mikroelektrod med etikettfria impedansmätningar. Det nya doseringssystemet ökar avsevärt dataflödet utan förlust i tidslösning.

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Biokemi Utgåva 156 G-proteinkopplad receptor (GPCR) impedans etikettfri cellbaserad analys koncentrationsresponsrelation agonist antagonist histamin
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