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Journal / Biochemistry / Label-Free Empedans Tabanlı GPCR Denemelerinde…
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Biochemistry

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JoVE Journal Biochemistry
Label-Free Empedans Tabanlı GPCR Denemelerinde Artırılmış İş İlerletme Protokolü
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Label-Free Empedans Tabanlı GPCR Denemelerinde Artırılmış İş İlerletme Protokolü

Article DOI: 10.3791/60686-v • 06:13 min • February 21st, 2020
February 21st, 2020 •
1, 1, 1, 1, 2, 2, 3, 3, 1,4
1Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 2Institute of Pharmacy, University of Regensburg, 3Department of Chemistry and Pharmacy, Friedrich-Alexander University Erlangen-Nuernberg, 4Fraunhofer Research Institution for Microsystems and Solid State Technologies EMFT

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Bu protokol, etiketsiz empedans ölçümleri kullanılarak tek bir mikroelektrot üzerinde yetiştirilen tek bir hücre tabakasından agonist kaynaklı GPCR aktivasyonu için tam doz-yanıt ilişkilerinin gerçek zamanlı kaydını göstermektedir. Yeni dosing şeması, zaman çözümlü zaman kaybı olmadan iş buzunu önemli ölçüde artırır.

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Biyokimya Sayı 156 G-protein birleştirilmiş reseptör (GPCR) empedans etiketsiz hücre bazlı test konsantrasyon-yanıt ilişkisi agonist antagonist histamin
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