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JoVE Journal Chemistry
Immobilize Metal Affinity Kromatografisinde Metal Yıkamanın Ölçülmesi
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Immobilize Metal Affinity Kromatografisinde Metal Yıkamanın Ölçülmesi

Article DOI: 10.3791/60690-v • 05:35 min • January 17th, 2020
January 17th, 2020 •
1, 1, 2, 1
1Department of Chemistry and Biochemistry, James Madison University, 2Biophysics Graduate Program, Ohio State University

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İmmobilize metal afinite kromatografisi kullanılarak hazırlanan numunelere tanıtılan metallerin kolay ölçülmesi için bir test salıyoruz. Yöntem, kolorimetrik metal göstergesi olarak hidroksinaphthol mavisi ve dedektör olarak UV-Vis spektrofotometresi kullanır.

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Kimya Sayı 155 immobilize metal afinite kromatografisi hidroksinaphthol mavisi HNB Ni-NTA metal kontaminasyonu Onun etiketi
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