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Journal / Chemistry / Kvantifiering av Metallurlakning i immobiliserad…
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JoVE Journal Chemistry
Kvantifiering av Metallurlakning i immobiliserad Metallaffinitetskromatografi
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Kvantifiering av Metallurlakning i immobiliserad Metallaffinitetskromatografi

Article DOI: 10.3791/60690-v • 05:35 min • January 17th, 2020
January 17th, 2020 •
1, 1, 2, 1
1Department of Chemistry and Biochemistry, James Madison University, 2Biophysics Graduate Program, Ohio State University

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Vi presenterar en analys för enkel kvantifiering av metaller som introduceras i prover som bereds med immobiliserad metallaffinitetskromatografi. Metoden använder hydroxynaphthol blått som den kolorimetriska metall indikatorn och en UV-VIS spektrofotometer som detektorn.

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Kemi immobiliserad metallaffinatografi hydroxynaphthol Blue HNB ni-NTA metallkontaminering his-tag
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