Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Isolation, Kultur og adipogen induktion af…
JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content.

JoVE Journal Developmental Biology
Isolation, Kultur og adipogen induktion af neuralcrest original fedt-afledte stamceller fra periaortisk fedtvæv
Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution
Journal (Developmental Biology)
Nuclei Isolation from Mouse Cardiac Progenitor Cells for Epigenome and Gene Expression Profiling at Single-Cell Resolution
Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids
Journal (Developmental Biology)
Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids
 
Click here for the English version

Isolation, Kultur og adipogen induktion af neuralcrest original fedt-afledte stamceller fra periaortisk fedtvæv

Article DOI: 10.3791/60691-v • 08:31 min • March 2nd, 2020
March 2nd, 2020 •
*1, *2, 3, 3, 4, 5, 4, 5, 1
1Department of Cardiology, Shanghai Chest Hospital, Shanghai Jiao Tong University, 2Shanghai Institute of Nutrition and Health, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 3Shanghai Key Laboratory of Hypertension, Shanghai Institute of Hypertension, Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 4Department of Cardiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 5Department of Cardiology, Shanghai Key Laboratory of Clinical Geriatric Medicine, Huadong Hospital Affiliated to Fudan University
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Vi præsenterer en protokol for isolation, kultur og adipogen induktion af neurale crest afledte stamceller (NCADSCs) fra det periaortiske fedtvæv af Wnt-1 Cre+/-; Rosa26RFP/+ mus. NCADSCs kan være en let tilgængelig kilde til Adscs til modellering adipogenesis eller lipogenese in vitro.

Tags

Udviklingsmæssige Biologi neurale crest celle Wnt-1 mus periaortisk fedtvæv fedt-afledte stromale celler stromale vaskulære fraktion cellekultur adipogenic induktion
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter