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通过四氯化碳诱导大鼠急性肝损伤 （CCl4） 通过奥罗胃管暴露
Chapters
Summary April 28th, 2020
Please note that all translations are automatically generated.
Click here for the English version.
该协议描述了通过CCl4暴露通过奥胃管诱导急性肝损伤（ALI）的一种常见和可行的方法。CCl4暴露在肝脏生物转化过程中通过活性氧物种的形成诱导 ALI。该方法用于分析ALI的病理生理学，并研究不同的肝保护策略。
Transcript
我想介绍一下我们关于通过四氯化碳通过四氯化碳通过胃管接触大鼠诱发急性肝损伤的工作。选择体重300至350克的成年雄性斯普拉格-道利大鼠。提供鼠周和水广告。
在上午 6 点到 12 点之间执行所有实验。以色列内盖夫本-古里安大学的动物护理喜剧批准了本协议中使用的所有治疗和测试程序。在22摄氏度的室温下保持大鼠12小时的灯光和12小时的暗循环。用 22 量表导管对尾静脉进行曲气。
在基线收集血液样本。血液生化分析包括血清GT、GPT和总胆红素的测量。在索罗卡医疗中心的生化实验室对肝酶和总胆红素水平进行了检查。
注意：接触高浓度的四氯化碳，包括蒸汽或皮肤吸收会影响中枢神经系统，使肝肾退化，长时间暴露可能导致昏迷或死亡。以一对一的比例准备 50% 的四氯化碳溶液与橄榄油混合为工具。通过四氯化碳管诱导体内的病理毒性。
通过大鼠的口腔插入一个肌管。让大鼠接触不同剂量的四氯化碳。轻度急性肝损伤每公斤1毫升，中度急性肝损伤每公斤2分5毫升，严重急性肝损伤每公斤5毫升。
对于假组，只让老鼠接触每公斤五毫升橄榄油。24小时从四氯化碳接触中采集血液样本。血液生化分析包括血清GT、GPT和总胆红素的测量。
将死老鼠放在解剖板上，将腹部朝上，剃掉腹部。使用手术刀，将从 Anus 到下巴的通风口全长。反射皮肤。
用手术刀，将腹壁从动脉切到西腓软骨，以暴露腹部内脏。将肝脏转移到培养皿中。进行赤氧树脂和 eosin 染色。
在显微镜下以 20 毫米的客观长度检查 200 倍的切片。肝脏部分应由专门的病理学家分级，对治疗方案视而不见。零分表示无肝异常，一至二表示轻度肝伤，三至四分表示中度肝伤，五至六分表示严重肝损伤。
不同时间的不同大鼠组显示在方案上。诱导急性肝损伤24小时后，GOT、GPT和胆红素总水平显著增加，取决于与对照组相比暴露的四氯化碳。P 值小于零点零零一。
在不同剂量的四氯化碳中毒后，所有组在24小时后观察到组织病理学的变化。所有三个介入组对对照组之间24小时组织学评分显著提高。P 值小于零点零五。
我们的研究小组介绍了通过四氯化碳通过四氯化碳接触，通过四氯化细胞接触大鼠诱发急性肝损伤的常见和物理方法。该方法用于研究急性肝损伤的病理生理学，并检验保护策略的不同性。非常感谢你的关注。
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