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Analyse de la consommation d'oxygène des primates non humains
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Analyse de la consommation d'oxygène des primates non humains

Article DOI: 10.3791/60696-v • 07:12 min • December 18th, 2019
December 18th, 2019 •
1, 2, 3, 1,4,5,6
1Department of Molecular Physiology and Biophysics, Vanderbilt University, 2Agilent Technologies, 3Department of Biological Sciences, Vanderbilt University, 4Department of Veterans Affairs Tennessee Valley, 5Department of Medicine, Vanderbilt University Medical Center, 6Department of Cell and Developmental Biology, Vanderbilt University

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Ce protocole démontre la mesure précise et reproductible de la consommation d'oxygène dans les îlots pancréatiques non-humains de primate. Les techniques de chargement des îfles et le revêtement de la microplaque fournissent un cadre pour une mesure efficace de la respiration dans d'autres types de sphéroïdes cultivés.

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Biologie du développement Numéro 154 islet consommation d'oxygène sphéroïde mitochondries cellules pancréas primates non humains
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