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JoVE Journal Developmental Biology
Analyse des Sauerstoffverbrauchs der nichtmenschlichen Primaten-Pankreas-Islet
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Analyse des Sauerstoffverbrauchs der nichtmenschlichen Primaten-Pankreas-Islet

Article DOI: 10.3791/60696-v • 07:12 min • December 18th, 2019
December 18th, 2019 •
1, 2, 3, 1,4,5,6
1Department of Molecular Physiology and Biophysics, Vanderbilt University, 2Agilent Technologies, 3Department of Biological Sciences, Vanderbilt University, 4Department of Veterans Affairs Tennessee Valley, 5Department of Medicine, Vanderbilt University Medical Center, 6Department of Cell and Developmental Biology, Vanderbilt University

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Dieses Protokoll demonstriert die genaue und reproduzierbare Messung des Sauerstoffverbrauchs in nichtmenschlichen Pankreasinseln. Die Beladetechniken der Isleten und die Beschichtung der Mikroplatte bieten einen Rahmen für eine effiziente Messung der Atmung in anderen Arten von kultivierten Sphäroiden.

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Entwicklungsbiologie Ausgabe 154 Islet Sauerstoffverbrauch Sphäroid Mitochondrien Zelle Bauchspeicheldrüse nicht-menschlicher Primat
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