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Journal / Biochemistry / Fuldt forarbejdet rekombinant KRAS4b: isolering…
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Biochemistry

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JoVE Journal Biochemistry
Fuldt forarbejdet rekombinant KRAS4b
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Fuldt forarbejdet rekombinant KRAS4b: isolering og karakterisering af det Farnesylerede og methylerede protein

Article DOI: 10.3791/60703-v • 07:08 min • January 16th, 2020
January 16th, 2020 •
1, 1, 1, 1, 1, 1, 1
1NCI RAS Initiative, Cancer Research Technology Program, Frederick National Laboratory for Cancer Research

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Prenylation er en vigtig modifikation på ydre membran bindende proteiner. Insekt celler kan manipuleres til fremstilling af farnesylerede og carboxymethylerede KRAS4b i mængder, der muliggør biofysiske målinger af protein-protein-og protein-lipid-interaktioner

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Biokemi produktion af insekt celle proteiner carboxymethylering farnesylering intakt SPEKTROMETRI naturlig massespektrometri overflade Plasmon resonans Liposomer
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