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Journal / Genetics / Universal og effektiv elektroporation protokol…
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Genetics

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JoVE Journal Genetics
Universal og effektiv elektroporation protokol for genteknologi af gastrointestinale organoider
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Universal og effektiv elektroporation protokol for genteknologi af gastrointestinale organoider

Article DOI: 10.3791/60704-v • 07:19 min • February 18th, 2020
February 18th, 2020 •
1, 1, 1, 1, 1, 1, 1
1Department of Visceral-, Thoracic and Vascular Surgery, University Hospital Carl Gustav Carus, Technische Universität Dresden

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Denne protokol beskriver en effektiv elektroporationsmetode til omladning af fire forskellige gastrointestinale organoidenheder med større plasmider (i det omfang 10 kB). Det kan udføres inden for en dag og behøver ikke omfattende forberedelse eller særlige, omkostningskrævende elektroporation buffere.

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Genetik elektroporation omladning CRISPR/Cas9 organoider genteknologi FACS PDAC CRC CCC gastrisk kræft
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