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Journal / Genetics / Universal och effektivt elektroporationprotokoll…
JoVE Journal
Genetics

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JoVE Journal Genetics
Universal och effektivt elektroporationprotokoll för genteknik av gastrointestinala organoider
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Universal och effektivt elektroporationprotokoll för genteknik av gastrointestinala organoider

Article DOI: 10.3791/60704-v • 07:19 min • February 18th, 2020
February 18th, 2020 •
1, 1, 1, 1, 1, 1, 1
1Department of Visceral-, Thoracic and Vascular Surgery, University Hospital Carl Gustav Carus, Technische Universität Dresden

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Detta protokoll beskriver en effektiv elektroporationmetod för transfection av fyra olika gastrointestinala organoidenheter med större plasmider (i den grad av 10 kB). Det kan utföras inom en dag och behöver inte omfattande förberedelse eller speciella, kostnadsintensiva elektroporation buffertar.

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Genetik Utgåva 156 elektroporation transfection CRISPR/Cas9 organoider genteknik FACS PDAC CRC CCC magcancer
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