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Journal / Genetics / LINE-1 Metyleringsanalyse i mesenchymale…
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Genetics
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JoVE Journal Genetics
LINE-1 Metyleringsanalyse i mesenchymale stamceller behandlet med osteosarkom-avledet ekstracellulære vesikler
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LINE-1 Metyleringsanalyse i mesenchymale stamceller behandlet med osteosarkom-avledet ekstracellulære vesikler

Article DOI: 10.3791/60705-v • 12:18 min • February 1st, 2020
February 1st, 2020 •
*1,2, *1,2, 1,2, 1,2
1Department of Oral and Maxillofacial Diseases, University of Helsinki, 2Helsinki University Hospital
* These authors contributed equally

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Beskrevet her er bruk av en metyleringsspesifikk probeforsterkningsmetode for å analysere metyleringsnivåer av LINE-1-elementer i mesenchymale stamceller behandlet med osteosarkom-avledede ekstracellulære vesikler. Ultracentrifugering, en populær prosedyre for å skille ekstracellulære vesikler fra føtal storfe serum, er også demonstrert.

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Genetikk Utgave 156 DNA-metylering LINE-1 epigenetikk ekstracellulære vesikler EV-utarmet fosterstorfeserum osteosarkom
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