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Encellet opløsning tredimensionel billeddannelse af intakte organoider
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Encellet opløsning tredimensionel billeddannelse af intakte organoider

Article DOI: 10.3791/60709-v • 10:40 min • June 5th, 2020
June 5th, 2020 •
*1,2,3, *1,2,3, 1,2,3, *1,2,3,4, *1,2,3
1Princess Máxima Center for Pediatric Oncology, 2Department of Cancer Research, Oncode Institute, Hubrecht Institute-KNAW Utrecht, 3Cancer Genomics Center (CGC), 4Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center (UMC) Utrecht
* These authors contributed equally

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Hele 3D-struktur og cellulære indhold af organoider, samt deres fænotopypiske lighed med det oprindelige væv kan fanges ved hjælp af encellede opløsning 3D-billeddannelse protokol beskrevet her. Denne protokol kan anvendes på en bred vifte af organoider varierende i oprindelse, størrelse og form.

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Biologi Problem 160 organoid encellet opløsning 3D-billeddannelse konfokal mikroskopi immunterapi optisk rydning
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