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Single-Cell Resolution Tredimensionell avbildning av intakta organoider
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Single-Cell Resolution Tredimensionell avbildning av intakta organoider

Article DOI: 10.3791/60709-v • 10:40 min • June 5th, 2020
June 5th, 2020 •
*1,2,3, *1,2,3, 1,2,3, *1,2,3,4, *1,2,3
1Princess Máxima Center for Pediatric Oncology, 2Department of Cancer Research, Oncode Institute, Hubrecht Institute-KNAW Utrecht, 3Cancer Genomics Center (CGC), 4Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences (KNAW) and University Medical Center (UMC) Utrecht
* These authors contributed equally

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Hela 3D-struktur och cellulära innehållet av organoider, liksom deras fenotypiska likhet med den ursprungliga vävnaden kan fångas med hjälp av encells upplösning 3D-bildframställning protokollet beskrivs här. Detta protokoll kan tillämpas på ett brett spektrum av organoider varierande ursprung, storlek och form.

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Biologi organoid encellig upplösning 3D-avbildning konfokalmikroskopi immunolabelling optisk clearing
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