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JoVE Journal Genetics
3D Multicolor DNA FISH Tool til at studere nuklear arkitektur i humane primære celler
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3D Multicolor DNA FISH Tool til at studere nuklear arkitektur i humane primære celler

Article DOI: 10.3791/60712-v • 11:25 min • January 25th, 2020
January 25th, 2020 •
1, 1, 1, 1
1Istituto Nazionale di Genetica Molecolare "Romeo ed Enrica Invernizzi", INGM

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3D multicolor DNA FISH repræsenterer et værktøj til at visualisere flere genomiske loci inden for 3D bevaret kerner, utvetydigt definere deres gensidige interaktioner og lokalisering i det nukleare rum på et enkelt celleniveau. Her beskrives en trinvis protokol for et bredt spektrum af humane primære celler.

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Genetik fluorescens in situ hybridisering 3D flerfarvet DNA FISH bevaret interfasenuclei nuklear arkitektur 3D genom foldning DNA-kontakter nukleare positionering menneskelige primære celler
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