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JoVE Journal Genetics
Herramienta 3D Multicolor DNA FISH para estudiar la arquitectura nuclear en células primarias humanas
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Herramienta 3D Multicolor DNA FISH para estudiar la arquitectura nuclear en células primarias humanas

Article DOI: 10.3791/60712-v • 11:25 min • January 25th, 2020
January 25th, 2020 •
1, 1, 1, 1
1Istituto Nazionale di Genetica Molecolare "Romeo ed Enrica Invernizzi", INGM

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3D multicolor DNA FISH representa una herramienta para visualizar múltiples loci genómicos dentro de núcleos conservados 3D, definiendo inequívocamente sus interacciones recíprocas y localización dentro del espacio nuclear a un solo nivel de célula. Aquí, se describe un protocolo paso a paso para un amplio espectro de células primarias humanas.

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Genética Número 155 hibridación flúor in situ ADN 3D FISH núcleos interfásicos preservados arquitectura nuclear plegado del genoma 3D contactos de ADN posicionamiento nuclear células primarias humanas
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