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Developmental Biology

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JoVE Journal Developmental Biology
Fluoreszierende Calcium-Bildgebung und anschließende In-Situ-Hybridisierung zur neuronalen Vorläufercharakterisierung bei Xenopus laevis
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Fluoreszierende Calcium-Bildgebung und anschließende In-Situ-Hybridisierung zur neuronalen Vorläufercharakterisierung bei Xenopus laevis

Article DOI: 10.3791/60726-v • 09:07 min • February 18th, 2020
February 18th, 2020 •
1, 2, 3, 4, 4, 5, 5, 2
1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Harvard University, 2Department of Biology, College of William and Mary, 3Harvard Medical School, Harvard University, 4Department of Bioengineering, California Institute of Technology, 5Department of Computer Science, College of William and Mary

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Wir präsentieren ein zweiteiliges Protokoll, das fluoreszierende Calcium-Bildgebung mit In-situ-Hybridisierung kombiniert, so dass der Experimentator Muster der Calciumaktivität mit Genexpressionsprofilen auf einzelzelligem Niveau korrelieren kann.

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Entwicklungsbiologie Ausgabe 156 Ca2+ Calcium Calcium-Bildgebung Calciumaktivität neuronale Entwicklung embryonale Entwicklung Neurotransmitter Neurowissenschaften
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