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Journal / Developmental Biology / Fluorescerande kalciumavbildning och…
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Developmental Biology

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JoVE Journal Developmental Biology
Fluorescerande kalciumavbildning och efterföljande in situ hybridisering för neuronal prekursor karakterisering i Xenopus laevis
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Fluorescerande kalciumavbildning och efterföljande in situ hybridisering för neuronal prekursor karakterisering i Xenopus laevis

Article DOI: 10.3791/60726-v • 09:07 min • February 18th, 2020
February 18th, 2020 •
1, 2, 3, 4, 4, 5, 5, 2
1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Harvard University, 2Department of Biology, College of William and Mary, 3Harvard Medical School, Harvard University, 4Department of Bioengineering, California Institute of Technology, 5Department of Computer Science, College of William and Mary

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Vi presenterar ett tvådelat protokoll som kombinerar fluorescerande kalciumavbildning med in situ hybridisering, vilket gör att experimentören att korrelera mönster av kalciumaktivitet med genuttryckprofiler på en cellnivå.

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Utvecklingsbiologi Utgåva 156 Ca2+ kalcium kalciumavbildning kalciumaktivitet neural utveckling embryonal utveckling signalsubstans neurovetenskap
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