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Journal / Immunology and Infection / TurboID-basert nærhetmerking for i…
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Immunology and Infection

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JoVE Journal Immunology and Infection
TurboID-basert nærhetmerking for i Planta-identifisering av proteinproteininteraksjonsnettverk
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TurboID-basert nærhetmerking for i Planta-identifisering av proteinproteininteraksjonsnettverk

Article DOI: 10.3791/60728-v • 07:02 min • May 17th, 2020
May 17th, 2020 •
*1, *2,3, 1, 1, 2, 2,3
1State Key Laboratory of Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, 2Department of Plant Biology, College of Biological Sciences, University of California, Davis, 3Genome Center, College of Biological Sciences, University of California, Davis
* These authors contributed equally

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Beskrevet her er en nærhet merking metode for identifisering av interaksjonpartnere av TIR-domenet til NLR immunreseptoren i Nicotiana benthamiana bladvev. Også gitt er en detaljert protokoll for identifisering av interaksjoner mellom andre proteiner av interesse ved hjelp av denne teknikken i Nikotiana og andre plantearter.

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Immunologi og infeksjon Utgave 159 nærhetmerking TurboID proteininteraksjoner desalting kvantifisering rensing TIR
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