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Journal / Genetics / Transkriptom-weite Profilierung von…
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JoVE Journal Genetics
Transkriptom-weite Profilierung von Protein-RNA-Wechselwirkungen durch Vernetzung und Immunpräzipitation durch FLAG-Biotin-Tandemreinigung
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Transkriptom-weite Profilierung von Protein-RNA-Wechselwirkungen durch Vernetzung und Immunpräzipitation durch FLAG-Biotin-Tandemreinigung

Article DOI: 10.3791/60730-v • 08:46 min • May 18th, 2020
May 18th, 2020 •
1,2, 1, 1
1Tsinghua-Peking Center for Life Sciences, School of Medicine and School of Life Sciences, Tsinghua University, 2Department of Molecular Biology, University of Texas Southwestern Medical Center

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Hier stellen wir ein modifiziertes CLIP-seq-Protokoll namens FbioCLIP-seq mit FLAG-Biotin-Tandemreinigung vor, um die RNA-Ziele von RNA-bindenden Proteinen (RBPs) in Säugetierzellen zu bestimmen.

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Genetik Ausgabe 159 FbioCLIP-seq RNA-Biologie RNA-bindendes Protein Protein-RNA-Interaktion WDR43 LIN28
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