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JoVE Journal Genetics
Profilazione a livello di trascrizione delle interazioni proteina-RNA attraverso il cross-linking e l'immunoprecipitazione mediata dalla purificazione tandem FLAG-Biotina
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Profilazione a livello di trascrizione delle interazioni proteina-RNA attraverso il cross-linking e l'immunoprecipitazione mediata dalla purificazione tandem FLAG-Biotina

Article DOI: 10.3791/60730-v • 08:46 min • May 18th, 2020
May 18th, 2020 •
1,2, 1, 1
1Tsinghua-Peking Center for Life Sciences, School of Medicine and School of Life Sciences, Tsinghua University, 2Department of Molecular Biology, University of Texas Southwestern Medical Center

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Qui presentiamo un protocollo CLIP-seq modificato chiamato FbioCLIP-seq con purificazione tandem FLAG-biotina per determinare gli obiettivi di RNA delle proteine leganti l'RNA (RRP) nelle cellule dei mammiferi.

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Genetica Numero 159 FbioCLIP-seq biologia dell'RNA proteina legante l'RNA interazione proteina-RNA WDR43 LIN28
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