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Journal / Immunology and Infection / 免疫荧光和定量PCR对白蝇组织中白蝇病毒的定位和定量…
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Immunology and Infection

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JoVE Journal Immunology and Infection
免疫荧光和定量PCR对白蝇组织中白蝇病毒的定位和定量
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免疫荧光和定量PCR对白蝇组织中白蝇病毒的定位和定量

Article DOI: 10.3791/60731-v • 06:25 min • February 8th, 2020
February 8th, 2020 •
1, 1, 1, 1, 1, 1
1Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogen and Insects, Institute of Insect Sciences, Zhejiang University

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我们描述了一种免疫荧光和定量PCR方法，用于昆虫组织中果子病毒的定位和定量。免疫荧光方案可用于共定病毒和载体蛋白。定量PCR协议可以扩展，以量化整个白蝇体内和受病毒感染的植物中的病毒。

Tags

免疫学和感染， 问题 156， 免疫荧光， 定量 PCR，贝米西亚塔巴奇，番茄黄叶卷曲病毒， 解剖， 原发唾液腺， 中肠
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