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Journal / Immunology and Infection / Lokalisering og kvantificering af Begomovirus i…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Lokalisering og kvantificering af Begomovirus i Whitefly Væv ved immunfluorescens og kvantitativ PCR
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Journal (Immunology and Infection)
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Lokalisering og kvantificering af Begomovirus i Whitefly Væv ved immunfluorescens og kvantitativ PCR

Article DOI: 10.3791/60731-v • 06:25 min • February 8th, 2020
February 8th, 2020 •
1, 1, 1, 1, 1, 1
1Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogen and Insects, Institute of Insect Sciences, Zhejiang University

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Vi beskriver en immunfluorescens og kvantitativ PCR-metode til lokalisering og kvantificering af begomovirus i insektvæv. Immunofluorescensprotokollen kan bruges til at colocalize virale og vektorproteiner. Den kvantitative PCR-protokol kan udvides til at kvantificere virus i hele whitefly organer og virus-inficerede planter.

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Immunologi og infektion immunfluorescens kvantitativ PCR Bemisia tabaci Tomat gul blad krølle virus,dissektion primær spytkirtel midgut
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