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Journal / Immunology and Infection / Lokalisatie en kwantificering van Begomovirussen…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Lokalisatie en kwantificering van Begomovirussen in Whitefly Weefsels door immunofluorescentie en kwantitatieve PCR
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Lokalisatie en kwantificering van Begomovirussen in Whitefly Weefsels door immunofluorescentie en kwantitatieve PCR

Article DOI: 10.3791/60731-v • 06:25 min • February 8th, 2020
February 8th, 2020 •
1, 1, 1, 1, 1, 1
1Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogen and Insects, Institute of Insect Sciences, Zhejiang University

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We beschrijven een immunofluorescentie en kwantitatieve PCR-methode voor de lokalisatie en kwantificering van begomovirussen in insectenweefsels. Het immunofluorescentieprotocol kan worden gebruikt om virale en vectoreiwitten samen te lokaliseren. Het kwantitatieve PCR-protocol kan worden uitgebreid om virussen in hele wittevlieglichamen en met virussen geïnfecteerde planten te kwantificeren.

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Immunologie en infectie Kwestie 156 immunofluorescentie kwantitatieve PCR Bemisia tabaci Tomaat geel blad krul virus,dissectie primaire speekselklier midgut
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