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Journal / Biochemistry / Berigelse af pattedyrvæv og Xenopus Oocytter med…
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Biochemistry

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JoVE Journal Biochemistry
Berigelse af pattedyrvæv og Xenopus Oocytter med kolesterol
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Berigelse af pattedyrvæv og Xenopus Oocytter med kolesterol

Article DOI: 10.3791/60734-v • 10:12 min • March 25th, 2020
March 25th, 2020 •
1, 1, 1, 1, 1, 2
1Department of Pharmacology, Addiction Science and Toxicology, The University of Tennessee HSC, 2Department of Chemistry, University of Illinois at Chicago

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To metoder til kolesterol berigelse præsenteres: anvendelse af cyclodextrin mættet med kolesterol til at berige pattedyr væv og celler, og brugen af kolesterol-beriget fosfolipid-baserede dispersioner (liposomer) til at berige Xenopus oocytter. Disse metoder er medvirkende til at bestemme virkningen af forhøjede kolesterolniveauer i molekylære, cellulære, og organfunktion.

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Biokemi kolesterol berigelse lipider cyclodextrin-kolesterol kompleks dispersion liposomer fosfolipider low-density lipoprotein LDL neuroner cerebralarterier Xenopus oocyttter kalium kanaler
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