Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Strategisk screening og karakterisering af Visual …
JoVE Journal
Biochemistry

This content is Open Access.

JoVE Journal Biochemistry
Strategisk screening og karakterisering af Visual GPCR-mini-G Protein Signaling Complex for vellykket krystallisering
Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening
Journal (Biochemistry)
Derivatization of Protein Crystals with I3C using Random Microseed Matrix Screening
Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers
Journal (Biochemistry)
Determination of In Vitro and Cellular Turn-on Kinetics for Fluorogenic RNA Aptamers
 
Click here for the English version

Strategisk screening og karakterisering af Visual GPCR-mini-G Protein Signaling Complex for vellykket krystallisering

Article DOI: 10.3791/60747-v • 09:19 min • March 16th, 2020
March 16th, 2020 •
1,2, 1, 3, 4, 4, 4, 1
1Laboratory of Biomolecular Research, Paul Scherrer Institute, 2Department of Biology, ETH Zürich, 3Center for Radiopharmaceutical Sciences, Paul Scherrer Institute, 4Laboratory of Molecular Biology, Medical Research Council

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Denne rapport beskriver screening af forskellige rengøringsmidler til forberedelse af den visuelle GPCR, rhodopsin, og dens kompleks med mini-Go. Biokemiske metoder, der karakteriserer kompleksets kvalitet på forskellige stadier under rensningen, påvises. Denne protokol kan generaliseres til andre membran protein komplekser for deres fremtidige strukturelle undersøgelser.

Tags

Biokemi vaskemiddel rensning rhodopsin-mini-Go kompleks stabilitet glycosylation retinal SEC UV-VIS spektroskopi SDS-PAGE LC-MS
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter