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JoVE Journal
Biochemistry

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视觉GPCR-迷你G蛋白信号复合物的战略筛选与表征,成功结晶
 
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视觉GPCR-迷你G蛋白信号复合物的战略筛选与表征,成功结晶

Article doi: 10.3791/60747
March 16th, 2020

Summary March 16th, 2020

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本报告描述了不同的洗涤剂的筛选,以准备视觉GPCR,红蛋白,其复合与微型Go。演示了在纯化过程中不同阶段对复杂质量进行特征的生化方法。该协议可以推广为其他膜蛋白复合物,用于其未来的结构研究。

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