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JoVE Journal Bioengineering
Wachstum von Human- und Schafhornhaut-Endothelzellschichten auf Biomaterialmembranen
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Wachstum von Human- und Schafhornhaut-Endothelzellschichten auf Biomaterialmembranen

Article DOI: 10.3791/60762-v • 05:20 min • February 6th, 2020
February 6th, 2020 •
1, 2, 1, 1,3,4,5,6,7,8, 1,3,4
1Queensland Eye Institute, 2King Faisal University, 3School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, 4Institute of Health and Biomedical Innovation, Queensland University of Technology, 5Science and Engineering Faculty, Queensland University of Technology, 6Faculty of Medicine, University of Queensland, 7Australian Institute for Bioengineering and Nanotechnology, University of Queensland, 8Faculty of Science, University of Western Australia

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Dieses Protokoll beschreibt die kritischen Schritte, die erforderlich sind, um hornhaut-Endothelzellkulturen aus Expflanzen von menschlichem oder Schafgewebe zu etablieren und zu züchten. Ein Verfahren zur Subkulierung von kornealen Endothelzellen auf membranösen Biomaterialien wird ebenfalls vorgestellt.

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Bioengineering Ausgabe 156 Hornhautendothel Hornhaut Descemet-Membran Explantation Biomaterialmembran Kulturkammer
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