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Biochemistry

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JoVE Journal Biochemistry
OaAEP1-Medieret enzymatisk syntese og immobilisering af polymeriseret protein til enkeltmolekylet spektroskopi
Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator
Journal (Biochemistry)
Dissipative Microgravimetry to Study the Binding Dynamics of the Phospholipid Binding Protein Annexin A2 to Solid-supported Lipid Bilayers Using a Quartz Resonator
2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
Journal (Biochemistry)
2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes
 
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OaAEP1-Medieret enzymatisk syntese og immobilisering af polymeriseret protein til enkeltmolekylet spektroskopi

Article DOI: 10.3791/60774-v • 08:34 min • February 5th, 2020
February 5th, 2020 •
1, 1, 1, 1, 1, 1, 1
1State Key Laboratory of Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University

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Her præsenterer vi en protokol til konjugere proteinmonomer af enzymer, der danner proteinpolymer med en kontrolleret sekvens og immobiliserer den på overfladen til enkeltmolekylets gennemspektroskopiundersøgelser.

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Biokemi enkeltmolekylets kraftspektroskopi biokonjugation proteinimmobilisering atomkraftsspektroskopi OaAEP1 proteinteknik
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