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Journal / Biology / Agrobacterium-Transformation d’embryon immature…
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Biology

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JoVE Journal Biology
Agrobacterium-Transformation d’embryon immature médié des lignées consanguines de maïs récalcitrants utilisant des gènes morphogènes
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Agrobacterium-Transformation d’embryon immature médié des lignées consanguines de maïs récalcitrants utilisant des gènes morphogènes

Article DOI: 10.3791/60782-v • 10:28 min • February 14th, 2020
February 14th, 2020 •
1,2, 1,3,4, 1,3, 1,3,5, 2, 2, 1,3
1Department of Agronomy, Iowa State University, 2Department of Applied Science and Technology, Corteva Agriscience, 3Crop Bioengineering Center, Iowa State University, 4Interdepartmental Plant Biology Major, Iowa State University, 5Interdepartmental Genetics and Genomics Major, Iowa State University

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Les gènes morphogènes végétaux peuvent être utilisés pour améliorer la transformation génétique des génotypes récalcitrants. Décrit ici est un protocole de transformation génétique Agrobacterium-négocié(QuickCorn) pour trois lignées de maïs de maïs public importants consanguins.

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Ce mois-ci à JoVE Numéro 156 Agrobacterium tumefaciens B73 Bbm transformation génétique Mo17 QuickCorn transgénique W22 Wus2 Zea mays
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