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JoVE Journal Bioengineering
Karakterisering single-molekyle konformationændringer under forskydning flow med fluorescens mikroskopi
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Karakterisering single-molekyle konformationændringer under forskydning flow med fluorescens mikroskopi

Article DOI: 10.3791/60784-v • 08:47 min • January 25th, 2020
January 25th, 2020 •
1, *2, *3, 3, 1,2, 1,4
1Department of Bioengineering, Lehigh University, 2Department of Materials Science and Engineering, Lehigh University, 3Department of Chemistry, Lehigh University, 4Department of Mechanical Engineering and Mechanics, Lehigh University
* These authors contributed equally

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Vi præsenterer en protokol for immobilisering af enkelte makromolekyler i mikrofluidiske enheder og kvantificereændringer i deres konformationer under forskydningsflow. Denne protokol er nyttig til at karakterisere biomekaniske og funktionelle egenskaber af biomolekyler såsom proteiner og DNA i et flowmiljø.

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Bioengineering von Willebrand faktor lambda DNA mikrofluidics forskydningflow TIRF konfokal fluorescens mikroskopi
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