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Karakteriseren single-molecule conformatieveranderingen onder schuifstroom met fluorescentiemicroscopie

Article DOI: 10.3791/60784-v • 08:47 min • January 25th, 2020
January 25th, 2020 •
1, *2, *3, 3, 1,2, 1,4
1Department of Bioengineering, Lehigh University, 2Department of Materials Science and Engineering, Lehigh University, 3Department of Chemistry, Lehigh University, 4Department of Mechanical Engineering and Mechanics, Lehigh University
* These authors contributed equally

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We presenteren een protocol voor het immobiliseren van enkele macromoleculen in microfluïde apparaten en het kwantificeren van veranderingen in hun conformaties onder schuifstroom. Dit protocol is nuttig voor het karakteriseren van de biomechanische en functionele eigenschappen van biomoleculen zoals eiwitten en DNA in een stroomomgeving.

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Bio-engineering von Willebrand factor lambda DNA microfluidics shear flow TIRF confocale fluorescentie microscopie
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