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JoVE Journal Immunology and Infection
Uma abordagem baseada em microscopia intravital para avaliar a permeabilidade intestinal e o desempenho do derramamento de células epiteliais
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Uma abordagem baseada em microscopia intravital para avaliar a permeabilidade intestinal e o desempenho do derramamento de células epiteliais

Article DOI: 10.3791/60790-v • 07:32 min • December 3rd, 2020
December 3rd, 2020 •
*1, *1, 1, 1, 1, 2, *1, *1, *1
1Medical Clinic 1, University of Erlangen-Nuremberg, University Hospital of Erlangen, 2Norwich Medical School, University of East Anglia, Norwich Research Park
* These authors contributed equally

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Aproveitando a microscopia intravital, o método aqui apresentado permite a visualização em tempo real do derramamento de células epiteliais intestinais em animais vivos. Portanto, a mucosa intestinal topicamente manchada (acriflavina e rhodamineB-dextran) de camundongos anesthetizados é imageda até a resolução unicelular usando microscopia confocal.

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Imunologia e Infecção Problema 166 derramamento de células permeabilidade intestino intestino vazamento microscopia intravital
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