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Journal / Developmental Biology / Visualisering og analyse af Pharyngeal Arch…
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Developmental Biology

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JoVE Journal Developmental Biology
Visualisering og analyse af Pharyngeal Arch Arterier ved hjælp af fuld-mount Immunohistochemistry og 3D Rekonstruktion
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Journal (Developmental Biology)
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Visualisering og analyse af Pharyngeal Arch Arterier ved hjælp af fuld-mount Immunohistochemistry og 3D Rekonstruktion

Article DOI: 10.3791/60797-v • 10:02 min • March 31st, 2020
March 31st, 2020 •
1,2, 1
1Department of Cell Biology and Molecular Medicine, New Jersey Medical School, Rutgers Biomedical and Health Sciences, 2Multidisciplinary PhD Program in Biomedical Sciences: Cell Biology, Neuroscience and Physiology Track, New Jersey Medical School, Rutgers Biomedical and Health Sciences

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Her beskriver vi en protokol til at visualisere og analysere svælg bue arterierne 3, 4 og 6 af mus embryoner ved hjælp af hele mount immunfluorescens, væv clearing, konfokal mikroskopi, og 3D rekonstruktion.

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Udviklingsmæssige Biologi endotelceller svælg bue arterie udvikling hele mount immunfluorescens væv clearing konfokal mikroskopi 3D rekonstruktion
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