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Journal / Cancer Research / Kombine Koşullu Knockdown ve Uyarlanmış Küre…
JoVE Journal
Cancer Research

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JoVE Journal Cancer Research
Kombine Koşullu Knockdown ve Uyarlanmış Küre Oluşumu Çalışma Hasta Kaynaklı Mide Kanseri Kök Hücrelerin bir Stemness-Associated Gene Çalışma
In Vitro Establishment of a Genetically Engineered Murine Head and Neck Cancer Cell Line using an Adeno-Associated Virus-Cas9 System
Journal (Cancer Research)
In Vitro Establishment of a Genetically Engineered Murine Head and Neck Cancer Cell Line using an Adeno-Associated Virus-Cas9 System
Identification and Characterization of Metastatic Factors by Gene Transfer into the Novel RIP-Tag; RIP-tva Murine Model
Journal (Cancer Research)
Identification and Characterization of Metastatic Factors by Gene Transfer into the Novel RIP-Tag; RIP-tva Murine Model
 
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Kombine Koşullu Knockdown ve Uyarlanmış Küre Oluşumu Çalışma Hasta Kaynaklı Mide Kanseri Kök Hücrelerin bir Stemness-Associated Gene Çalışma

Article DOI: 10.3791/60799-v • 09:24 min • May 9th, 2020
May 9th, 2020 •
*1, *1, *1, 1
1Guangdong Key Laboratory of Genome Stability and Human Disease Prevention, Department of Pharmacology and Shenzhen University International Cancer Center, Shenzhen University School of Medicine
* These authors contributed equally

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Bu protokolde, clusterin'in hasta kaynaklı GCSC'lerin sapı üzerindeki etkisini incelemek için koşullu bir nakavt sistemi ve uyarlanmış küre oluşum testini kullanarak deneysel bir tasarım salıyoruz. Protokol, farklı CSC türlerinde köklüğe bağlı genlerin hem in vitro hem de in vivo fonksiyonlarını incelemek için kolayca uyarlanabilir.

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Kanser Araştırma Sayı 159 mide kanseri kök hücre koşullu nakavt küre oluşumu tetkik clusterin kök
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