Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Gebruik van gevriesde embryo's voor…
JoVE Journal
Genetics

A subscription to JoVE is required to view this content.

JoVE Journal Genetics
Gebruik van gevriesde embryo's voor hoogrendementproductie van genetisch gemodificeerde muizen
Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
Journal (Genetics)
Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection
Journal (Genetics)
Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection
 
Click here for the English version

Gebruik van gevriesde embryo's voor hoogrendementproductie van genetisch gemodificeerde muizen

Article DOI: 10.3791/60808-v • 06:46 min • April 2nd, 2020
April 2nd, 2020 •
*1,2,3, *4,5, 6,7, 8,9,10, 8,11, 3, 8,9,10,12,13, 1
1Max Planck Florida Institute for Neuroscience, 2Life Science Research Center, University of Toyama, 3Department of Biochemical Engineering, Graduate School of Science and Engineering, Yamagata University, 4Graduate School of Innovative Life Science, University of Toyama, 5Department of Biochemistry, Faculty of Pharmacy, Cairo University, 6Division of Regenerative Medicine, Center for Molecular Medicine, Jichi Medical University, 7Division of Stem Cell Research and Drug Development, Center for Development of Advanced Medical Technology, Jichi Medical University, 8Synthetic Biology Division, Research Center for Advanced Science and Technology, University of Tokyo, 9Institute for Advanced Biosciences, Keio University, 10Graduate School of Media and Governance, Keio University, 11Department of Molecular Oncology, Graduate School of Medicine, Chiba University, 12Department of Biological Sciences, School of Science, University of Tokyo, 13College of Arts and Sciences, University of Tokyo
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Hier presenteren we een aangepaste methode voor cryopreservatie van eencellige embryo's, evenals een protocol dat het gebruik van gevriesontdooide embryo's en elektroporatie koppelt voor de efficiënte generatie van genetisch gemodificeerde muizen.

Tags

Genetica Nummer 158 CRISPR/Cas9 eencellige embryo cryopreservatie elektroporatie genetisch gemodificeerde muizen GM genoombewerking FBS
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter