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Journal / Biochemistry / 免疫蛍光を用いたTNBS誘発クローン病を用いたマウスの結腸におけるエストロゲン受容体の可視化…
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Biochemistry

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JoVE Journal Biochemistry
免疫蛍光を用いたTNBS誘発クローン病を用いたマウスの結腸におけるエストロゲン受容体の可視化
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Journal (Biochemistry)
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Combining Raman Imaging and Multivariate Analysis to Visualize Lignin, Cellulose, and Hemicellulose in the Plant Cell Wall
Journal (Biochemistry)
Combining Raman Imaging and Multivariate Analysis to Visualize Lignin, Cellulose, and Hemicellulose in the Plant Cell Wall
 
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免疫蛍光を用いたTNBS誘発クローン病を用いたマウスの結腸におけるエストロゲン受容体の可視化

Article DOI: 10.3791/60813-v • 08:02 min • March 12th, 2020
March 12th, 2020 •
1, 2, 3, 2, 1
1Department of Cytobiochemistry, Faculty of Biology and Environmental Protection, University of Lodz, 2Department of Biochemistry, Faculty of Medicine, Medical University of Lodz, 3Laboratory of Microscopic Imaging and Specialized Biological Techniques, Faculty of Biology and Environmental Protection, University of Lodz

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このプロトコルは、クローン病の完全に検証されたTNBS誘導マウスモデルと、パラフィンに埋め込まれたホルマリン固定結腸切片の免疫蛍光を用いた免疫体化学によるエストロゲン受容体の視覚化方法を提示する。

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生化学、問題157、クローン病、マウスモデル、エストロゲン受容体、Gタンパク質結合エストロゲン受容体、GPER、ERα、ERβ、免疫神学的化学、共焦点顕微鏡
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