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JoVE Journal Biochemistry
Visualisering av östrogenreceptorer i kolon av möss med TNBS-inducerad Crohns sjukdom med immunfluorescens
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Visualisering av östrogenreceptorer i kolon av möss med TNBS-inducerad Crohns sjukdom med immunfluorescens

Article DOI: 10.3791/60813-v • 08:02 min • March 12th, 2020
March 12th, 2020 •
1, 2, 3, 2, 1
1Department of Cytobiochemistry, Faculty of Biology and Environmental Protection, University of Lodz, 2Department of Biochemistry, Faculty of Medicine, Medical University of Lodz, 3Laboratory of Microscopic Imaging and Specialized Biological Techniques, Faculty of Biology and Environmental Protection, University of Lodz

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Protokollet presenterar en komplett validerad TNBS-inducerad murine modell av Crohns sjukdom och metoder för visualisering av östrogenreceptorer av immunohistochemistry med immunofluorescens av formalin-fast kolon sektioner inbäddade i paraffin.

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Biokemi Crohns sjukdom murine modell östrogenreceptorer G protein-kopplade östrogenreceptor GPER ERα ERβ immunohistochemistry konfokal mikroskopi
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