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Journal / Biochemistry / Visualisering af østrogenreceptorer i koloner af…
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Biochemistry

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JoVE Journal Biochemistry
Visualisering af østrogenreceptorer i koloner af mus med TNBS-induceret Crohns sygdom ved hjælp af immunofluorescens
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Journal (Biochemistry)
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Visualisering af østrogenreceptorer i koloner af mus med TNBS-induceret Crohns sygdom ved hjælp af immunofluorescens

Article DOI: 10.3791/60813-v • 08:02 min • March 12th, 2020
March 12th, 2020 •
1, 2, 3, 2, 1
1Department of Cytobiochemistry, Faculty of Biology and Environmental Protection, University of Lodz, 2Department of Biochemistry, Faculty of Medicine, Medical University of Lodz, 3Laboratory of Microscopic Imaging and Specialized Biological Techniques, Faculty of Biology and Environmental Protection, University of Lodz

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Protokollen præsenterer en komplet valideret TNBS-induceret murine model af Crohns sygdom og metoder til visualisering af østrogen receptorer ved immunhistokemi ved hjælp af immunofluorescens af formalin-faste kolon sektioner indlejret i paraffin.

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Biokemi Crohns sygdom murine model østrogen receptorer G protein-koblet østrogen receptor GPER ERα ERβ immunhistokemi konfokal mikroskopi
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