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Journal / Biochemistry / İmmünofluoresans kullanarak TNBS'ye Bağlı Crohn…
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Biochemistry

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JoVE Journal Biochemistry
İmmünofluoresans kullanarak TNBS'ye Bağlı Crohn Hastalığı olan Farelerin Kolonlarında Östrojen Reseptörlerinin Görüntülenmesi
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İmmünofluoresans kullanarak TNBS'ye Bağlı Crohn Hastalığı olan Farelerin Kolonlarında Östrojen Reseptörlerinin Görüntülenmesi

Article DOI: 10.3791/60813-v • 08:02 min • March 12th, 2020
March 12th, 2020 •
1, 2, 3, 2, 1
1Department of Cytobiochemistry, Faculty of Biology and Environmental Protection, University of Lodz, 2Department of Biochemistry, Faculty of Medicine, Medical University of Lodz, 3Laboratory of Microscopic Imaging and Specialized Biological Techniques, Faculty of Biology and Environmental Protection, University of Lodz

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Protokol, Crohn hastalığının tam doğrulanmış TNBS kaynaklı murine modelini ve parafine gömülü formalin-sabit kolon bölümlerinin immünororesansını kullanarak immünohistokimya ile östrojen reseptörlerinin görüntülenmesi için kullanılan yöntemleri sunar.

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Biyokimya Sayı 157 Crohn hastalığı murine modeli östrojen reseptörleri G protein-birleşen östrojen reseptörü GPER ERα ERβ immünohistokimya konfokal mikroskopi
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