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Journal / Genetics / Stable Knockdown of Genes Encoding Extracellular…
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JoVE Journal Genetics
Stable Knockdown of Genes Encoding Extracellular Matrix Proteins nella linea cell Myoblast C2C12 Utilizzando Small-Hairpin (sh)RNA
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Stable Knockdown of Genes Encoding Extracellular Matrix Proteins nella linea cell Myoblast C2C12 Utilizzando Small-Hairpin (sh)RNA

Article DOI: 10.3791/60824-v • 12:19 min • February 12th, 2020
February 12th, 2020 •
1, 1, 1
1Orthopaedic Research Laboratories, Leni & Peter W. May Department of Orthopaedics, Icahn School of Medicine at Mount Sinai

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Forniamo un protocollo per abbattere stabilmente i geni che codificano le proteine eCM (Extracellular Matrix) nei mioblasti C2C12 usando l'RNA per piccoli capellini (sh). Mirando ad ESEMPIO ad ADAMTSL2, descriviamo i metodi per la convalida dell'efficienza di abbattimento a livello mRNA, proteina e cellulare durante il mioblasto C2C12 alla differenziazione del miotubo.

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Genetica Numero 156 Proteasi ADAMTS proteine simili ad ADAMTS mioblasti C2C12 matrice extracellulare differenziazione muscolare scheletrica miogenesi shRNA
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