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Journal / Genetics / Stabiler Knockdown von Genen, die extrazelluläre…
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Genetics

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JoVE Journal Genetics
Stabiler Knockdown von Genen, die extrazelluläre Matrixproteine in der C2C12 Myoblast-Zelllinie mit Small-Hairpin (sh)RNA kodieren
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Stabiler Knockdown von Genen, die extrazelluläre Matrixproteine in der C2C12 Myoblast-Zelllinie mit Small-Hairpin (sh)RNA kodieren

Article DOI: 10.3791/60824-v • 12:19 min • February 12th, 2020
February 12th, 2020 •
1, 1, 1
1Orthopaedic Research Laboratories, Leni & Peter W. May Department of Orthopaedics, Icahn School of Medicine at Mount Sinai

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Wir bieten ein Protokoll, um Gene, die extrazelluläre Matrixproteine (ECM) in C2C12-Myoblasten kodieren, mit Small-Hairpin (sh) RNA zu fallig zu senken. Als Beispiel für ADAMTSL2 beschreiben wir die Methoden zur Validierung der Knockdown-Effizienz auf mRNA-, Protein- und Zellebene während der Myotube-Differenzierung von C2C12.

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Genetik Ausgabe 156 ADAMTS-Proteasen ADAMTS-ähnliche Proteine C2C12-Myoblasten extrazelluläre Matrix Skelettmuskeldifferenzierung Myogenese shRNA
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