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Immunology and Infection

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JoVE Journal Immunology and Infection
Identificazione delle trappole extracellulari neutrophil in Paraffin-Embedded Feline Arterial Thrombi utilizzando la microscopia immunofluorescenza
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Identificazione delle trappole extracellulari neutrophil in Paraffin-Embedded Feline Arterial Thrombi utilizzando la microscopia immunofluorescenza

Article DOI: 10.3791/60834-v • 08:48 min • March 29th, 2020
March 29th, 2020 •
1, 2, 3, 2
1Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, Davis, 2Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, 3Department of Veterinary Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis

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Descriviamo un metodo per identificare le trappole extracellulari neutrofiche (NET) nei trombi arteriosi cardiogenici felini fissi e incorporati in parina di formaldeide utilizzando il recupero di antigeni indotti dal calore e un doppio protocollo di immunoetichettatura.

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Immunologia e infezione numero 157 microscopia immunofluorescenza istone citrullinato H3 elastasi neutrofila trombosi arteriosa cardiomiopatia ipertrofica felina
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