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Journal / Immunology and Infection / Identifikation af neutrofile ekstracellulære…
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Immunology and Infection

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JoVE Journal Immunology and Infection
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Identifikation af neutrofile ekstracellulære fælder i paraffin-embedded feline arteriel trombose ved hjælp af Immunofluorescence Mikroskopi

Article DOI: 10.3791/60834-v • 08:48 min • March 29th, 2020
March 29th, 2020 •
1, 2, 3, 2
1Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, Davis, 2Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, 3Department of Veterinary Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis

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Vi beskriver en metode til at identificere neutrofile ekstracellulære fælder (NETs) i formaldehyd-faste og paraffin-indlejret feline kardiogene trombi ved hjælp af varme-induceret antigen hentning og en dobbelt immunmærkning protokol.

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Immunologi og infektion Problem 157 mikroskopi af immunfluorescens citrullinslået histon H3 neutrofil elastase arteriel trombose feline hypertrofisk kardiomyopati
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