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Journal / Immunology and Infection / Identificatie van Neutrophil Extracellular Traps…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Identificatie van Neutrophil Extracellular Traps in Paraffine-Embedded Feline Arterial Thrombi using Immunofluorescentie Microscopie
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Identificatie van Neutrophil Extracellular Traps in Paraffine-Embedded Feline Arterial Thrombi using Immunofluorescentie Microscopie

Article DOI: 10.3791/60834-v • 08:48 min • March 29th, 2020
March 29th, 2020 •
1, 2, 3, 2
1Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, Davis, 2Department of Veterinary Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, 3Department of Veterinary Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis

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We beschrijven een methode om neutrofiele extracellulaire vallen (NET's) te identificeren in formaldehyde-vaste en paraffine-ingebedde katachtige cardiogene trombi met behulp van warmte-geïnduceerde antigen retrieval en een dubbele immunolabeling protocol.

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Immunologie en infectie kwestie 157 immunofluorescentie microscopie gecitrulute histone H3 neutrofiele elastase arteriële trombose feline hypertrofische cardiomyopathie
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