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Journal / Developmental Biology / Effektiv differentiering af postganglioniske…
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Developmental Biology
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JoVE Journal Developmental Biology
Effektiv differentiering af postganglioniske sympatiske neuroner ved hjælp af humane pluripotente stamceller under Feeder-fri og kemisk definerede kulturbetingelser
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Effektiv differentiering af postganglioniske sympatiske neuroner ved hjælp af humane pluripotente stamceller under Feeder-fri og kemisk definerede kulturbetingelser

Article DOI: 10.3791/60843-v • 10:24 min • May 24th, 2020
May 24th, 2020 •
1,2, 1,2,3
1Center for Molecular Medicine, University of Georgia, 2Department of Biochemistry and Molecular Biology, Franklin College of Arts and Sciences, University of Georgia, 3Department of Cellular Biology, Franklin College of Arts and Sciences, University of Georgia

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I denne protokol beskriver vi en stabil, yderst effektiv differentieringsstrategi for generering af postganglioniske sympatiske neuroner fra menneskelige pluripotente stamceller. Denne model vil gøre neuroner til rådighed for brug af undersøgelser af flere autonome lidelser.

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Udviklingsbiologi menneskelige pluripotente stamceller neurale crest sympatiske neuroner autonome nervesystem sympatisk nervesystem feeder-fri kemisk defineret stamcellekultur
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