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Journal / Developmental Biology / Differenziazione efficiente dei neuroni simpatici …
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Developmental Biology
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JoVE Journal Developmental Biology
Differenziazione efficiente dei neuroni simpatici postganglionici utilizzando cellule staminali pluripotenti umane in condizioni di coltura senza alimentatore e definite chimicamente
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Differenziazione efficiente dei neuroni simpatici postganglionici utilizzando cellule staminali pluripotenti umane in condizioni di coltura senza alimentatore e definite chimicamente

Article DOI: 10.3791/60843-v • 10:24 min • May 24th, 2020
May 24th, 2020 •
1,2, 1,2,3
1Center for Molecular Medicine, University of Georgia, 2Department of Biochemistry and Molecular Biology, Franklin College of Arts and Sciences, University of Georgia, 3Department of Cellular Biology, Franklin College of Arts and Sciences, University of Georgia

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In questo protocollo, descriviamo una strategia di differenziazione stabile e altamente efficiente per la generazione di neuroni simpatici postganglionici dalle cellule staminali pluripotenti umane. Questo modello renderà i neuroni disponibili per l'uso di studi di disturbi autonomici multipli.

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Biologia dello sviluppo numero 159 cellule staminali pluripotenti umane cresta neurale neuroni simpatici sistema nervoso autonomo sistema nervoso simpatico coltura di cellule staminali chimicamente definite
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