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Overvågning af eIF4F-samling ved måling af eIF4E-eIF4G-interaktion i levende celler
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Overvågning af eIF4F-samling ved måling af eIF4E-eIF4G-interaktion i levende celler

Article DOI: 10.3791/60850-v • 08:47 min • May 1st, 2020
May 1st, 2020 •
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1p53 Laboratory, Neuros/Immunos, A*STAR (Agency for Science, Technology and Research)

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Her præsenterer vi en protokol til måling af eIF4E-eIF4G-interaktion i levende celler, der vil gøre det muligt for brugeren at evaluere lægemiddelinduceret forstyrrer af eIF4F-kompleks dynamik i screeningsformater.

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Kræftforskning Problem 159 Cellulær biologi eIF4F-kompleks Translationel kontrol Luciferase komplementationsanalyse mTOR-signalering proteinproteininteraktioner
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