JoVE Journal
Developmental Biology
Developmental Biology
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微挥和荧光素-提拉美化物,用于小鼠肾上腺的多重免疫染色 – 使用来自同一宿主物种的未结合原抗体
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Article
DOI:
10.3791/60868-v
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07:37 min
•
February 21st, 2020
微挥和荧光素-提拉美化物,用于小鼠肾上腺的多重免疫染色 – 使用来自同一宿主物种的未结合原抗体
February 21st, 2020
•
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
利用来自同一宿主物种的初级抗体,已经建立了几种不同的多路免疫染色方法。在这里,我们描述了在正式固定石蜡嵌入小鼠肾上腺部分进行多倍免疫染色期间,使用微波介导的抗体剥离和荧光素-酪酰胺来阻止抗体的交叉反应。