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JoVE Journal Cancer Research
Forbedret levedyktighet for Ex vivo 3D hydrogelkulturer av pasientavledede Xenografts i en perfused mikrofluidisk plattform
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Forbedret levedyktighet for Ex vivo 3D hydrogelkulturer av pasientavledede Xenografts i en perfused mikrofluidisk plattform

Article DOI: 10.3791/60872-v • 12:12 min • December 5th, 2020
December 5th, 2020 •
1, 2, 3, 4, 1,5,6, 1,7, 2, 3, 1,5,6
1Department of BioSciences, Rice University, 2Mimetas US Inc, 3Department of Genitourinary Medical Oncology Research, Division of Cancer Medicine, The University of Texas MD Anderson Cancer Center, 4Mimetas B.V., 5Department of Bioengineering, Rice University, 6Department of Diagnostic and Biomedical Sciences, The University of Texas Health Science Center, 7Sheikh Ahmed Center for Pancreatic Cancer Research, The University of Texas MD Anderson Cancer Center

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Denne protokollen demonstrerer metoder for å muliggjøre utvidet in vitro kultur av pasientavledede xenografts (PDX). Ett trinn forbedrer den generelle levedyktigheten til flercellede klyngekulturer i 3D-hydrogeler, gjennom enkel fjerning av ikke-levedyktige enkeltceller. Et sekundært trinn viser beste praksis for PDX-kultur i en perfundert mikrofluidisk plattform.

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Kreftforskning utgave 166 3D-kultur hydrogeler mikrofluidier pasientavledede xenografter prostatakreft in vitro kreftmodeller høy gjennomstrømningsbehandling perfusjon
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